Science Lab - Phycocyanin

Introduction\nPhycocyanin(PC) is a gamey photopigment used for many applications, for instance as a inseparable intellectual nourishment colorant in the food industry (e.g. M&M and Gatorade). This pigment is establishd by a natural mutant of the carmine micro algae G. Sulphuraria ( taste 074G). This kind of algae shadow germinate in several(a) situations. It can produce in the dark, on various total compounds and at various Ph. values. Phycocyanin is prep be only in cyanobacteria, class Cryptophyceae and Rhodophyceae, is among the most important substances produced unequivocally in cyanobacterial and microalgae cultures. The mercantile employment of PC is make in phototrophic cultures of the cyanobacterium Spirulina plantensis. The energy reference book for this is sunlight. Because these cultures depend on the sunlight, or externally supplied light it is truly hard to scale up these cultures without losing productivity. This is because the surface area to majority r atio decreases at increase scale and the light paths inside(a) the cultures get longer. This causes self-shading and dark and this develops bootless zones. (Eriksen J. K., 2005)\nThe algae Galdieria Sulphuraria, can grow photo-, mixo and heterotrophically in warm acid springs and can still produce PC. As told before the strain 047 G of G. sulphuraria does even so when it is grown heterotrophically in darkness. (Schnarrenberger, 1995) risque rates of biomass which is possible in high- prison cell-density fed-batch cultures of G. sulphuraria 074G compensates for the relative low PC concentration in the algae; 3-4 mg g-1 of biomass dry pitch in comparison to the phototrophic Spirulina platensis; 60-74 mg g-1 dry weight. Altough there is a big difference betwixt the amount of PC per cell the total production of G. Sulphuraria 074G is 1.7-13.6 quantify higher than the rate of PC production found in outdoor S. platensis cultures. (Materassi, 1997) Following those findings we are go ing to look whether the production of PC by G. Sulphuraria 074G is appl...